Peptide Dosage Calculations & Reconstitution  Instructions


Why Use a Peptide Reconstitution Calculator?


Accurate peptide dosing is the foundation of effective peptide therapy. A single miscalculation can result in underdosing (wasting your investment and missing therapeutic benefits) or overdosing (risking unnecessary side effects). Our peptide reconstitution calculator eliminates guesswork and ensures precision every time you prepare a vial.


Whether you're working with BPC-157, TB-500, Ipamorelin, CJC-1295, or any other research peptide, this calculator provides instant, accurate dosing information tailored to your specific vial size, reconstitution volume, and target dose.



Peptide Calculator tool for Reconstitution

Quickly calculate the correct dosage and injection volume based on your vial strength, reconstitution volume, and desired dose per injection.


How to Use the calculator tool:


  1. Enter total peptide amount – usually listed on the vial (e.g., 5 mg).
  2. Choose reconstitution volume – how many mL of bacteriostatic water you'll add (e.g., 2 mL).
  3. Set the desired dose per injection, in micrograms (mcg), based on your protocol.
  4. Get Results:
  • Micrograms per IU
  • IU needed per dose
  • Number of total doses in the vial
  • Visual graph of dosing over time

Peptide Dosage Calculator










Results:

⚠️ Disclaimer: This calculator is for research and educational purposes only. It does not replace professional medical advice. Always consult a healthcare provider before starting peptide protocols.

Peptide Units Calculation Explained


Each 1 mL insulin syringe is marked with 100 units, so 1 unit = 0.01 mL. The number of units to draw depends on how much liquid (bacteriostatic water) is added to the vial and the desired dose.


Basic Steps


  • Add up how much peptide is in the vial (mg or mcg) and how much water (mL) you mix in.
  • To find out how much solution contains your desired dose:
  • Divide the total peptide (mcg) by the amount of water (mL) to get the concentration.
  • Divide your desired dose (mcg) by the concentration to get the volume (mL) needed.
  • Multiply that volume by 100 to get the number of units on the syringe.


Example


Suppose:

  • 5 mg peptide (5,000 mcg) is in the vial
  • 2 mL bacteriostatic water added
  • You want to inject 250 mcg
  1. Concentration = 5,000 mcg / 2 mL = 2,500 mcg/mL
  2. Volume needed = 250 mcg / 2,500 mcg/mL = 0.1 mL
  3. 0.1 mL = 10 units on an insulin syringe

So, pull the syringe to the 10-mark for a 250 mcg dose.


Common Reconstitution Volumes


Recommended Reconstitution Volumes


While you can technically use any volume of bacteriostatic water, certain amounts work better for different scenarios:


  • 2ml (Most Popular): The ideal balance for most peptides. Provides manageable concentration while keeping injection volumes reasonable. Works well for doses between 100-500mcg.


  • 1ml (High Concentration): Best when you want minimal injection volume or are taking very small doses. Can make precise measurements more challenging for beginners.


  • 3ml (Lower Concentration): Optimal for micro-dosing protocols or when you need very precise control over small dose adjustments. Results in larger injection volumes.


For most users working with standard 5mg vials, we recommend starting with 2ml of bacteriostatic water.


Peptide-Specific Dosing Guidelines


Use these general guidelines as starting points. Always consult with a healthcare provider before beginning any peptide protocol.


BPC-157 (Body Protection Compound)

  • Typical dose: 250-500mcg
  • Frequency: 1-2 times daily
  • Standard vial: 5mg


TB-500 (Thymosin Beta-4)

  • Typical dose: 2-2.5mg
  • Frequency: 2-3 times per week
  • Standard vial: 5mg or 10mg


Ipamorelin

  • Typical dose: 200-300mcg
  • Frequency: 2-3 times daily
  • Standard vial: 5mg


CJC-1295 (with or without DAC)

  • Typical dose: 100-200mcg
  • Frequency: 1-3 times per week (with DAC), daily (without DAC)
  • Standard vial: 2mg or 5mg


Sermorelin

  • Typical dose: 200-500mcg
  • Frequency: Once daily before bed
  • Standard vial: 5mg or 15mg


Note: These are general reference ranges. Individual protocols vary based on goals, body weight, and response.


Troubleshooting Common Issues

  • My calculator result shows more units than my syringe holds
    You've likely used too much bacteriostatic water for your desired dose. Reconstitute with a smaller volume (try 1ml instead of 2ml) to create a more concentrated solution.
  • The numbers seem too small (2-3 units)
    Very small measurements are difficult to draw accurately. Consider using less water during reconstitution to achieve a higher concentration, making your target dose correspond to a larger, more measurable number of units.
  • I'm getting different results from different calculators
    Verify that all inputs are in the same units. Check that you're comparing calculations for the same reconstitution volume and syringe type. If discrepancies persist, work through the math manually using our formula above.
  • My reconstituted peptide looks cloudy
    This isn't a calculation issue, but it indicates a problem. Peptide solutions should be clear. Cloudiness may indicate contamination, incorrect reconstitution, or degraded peptide. Do not use cloudy solutions.
  • I forgot how much water I added to my vial
    Unfortunately, you cannot accurately calculate dosing without knowing the reconstitution volume. It's safer to discard the vial and start fresh with documented measurements than to risk incorrect dosing.

5. Storage and Handling Best PracticesPeptide Storage Guidelines


Proper storage is essential for maintaining peptide potency and ensuring your calculated doses remain accurate.

  • Before Reconstitution:
  • Store lyophilized peptides in the freezer (-20°C or colder)
  • Keep away from light and moisture
  • Properly stored, most peptides remain stable for 1-2 years
  • After Reconstitution:
  • Refrigerate immediately (2-8°C)
  • Use bacteriostatic water to extend stability
  • Most peptides remain potent for 28 days when properly refrigerated
  • Protect from light using amber vials or foil wrap
  • Never freeze reconstituted peptides
  • Label Your Vials:
    Always mark your vial with:
  • Reconstitution date
  • Bacteriostatic water volume used
  • Concentration (mg/ml)
  • Units to draw per dose
  • Expiration date (28 days from reconstitution)


Important Safety Information

Sterile Technique is Essential


Even with perfect calculations, improper handling can lead to contamination and serious infections. Always use:


  • New, sterile needles and syringes
  • Alcohol wipes for vial tops and injection sites
  • Proper hand hygiene
  • Clean work surfaces


When to Seek Help


Stop using peptides and contact your healthcare provider if you experience:

  • Severe injection site reactions (excessive swelling, redness, warmth)
  • Allergic reactions (rash, difficulty breathing, swelling)
  • Unexpected side effects
  • Symptoms that worsen rather than improve


Quality Matters


Calculations only work when your peptides contain the labeled amount of active compound. Always source peptides from reputable suppliers who provide:


  • Third-party testing certificates (COA)
  • Proper labeling and documentation
  • Appropriate storage during shipping
  • Clear expiration dates



Need Help?
Have questions about using this calculator or peptide therapy in general? Contact us or consult with a qualified healthcare provider experienced in peptide therapy.


Learn More About Accurate Peptide Dosing:

  • Can I use regular sterile water instead of bacteriostatic water?

    You can, but sterile water significantly reduces vial stability after reconstitution. With sterile water, peptides typically remain stable for only 3-5 days versus 28 days with bacteriostatic water. This means more waste and more frequent reconstitution.

  • What syringe size should I use?

    Most peptide users rely on insulin syringes:

    • 1ml (100 unit) syringes: Most versatile, works for most protocols
    • 0.5ml (50 unit) syringes: Better for very small doses, offers finer gradations
    • 0.3ml (30 unit) syringes: Best for micro-dosing protocols

  • How do I know if my peptides are still good?

    Look for:

    • Clear, colorless solution (cloudiness indicates problems)
    • No unusual odor
    • Within 28 days of reconstitution
    • Stored properly in refrigerator
    • Continued therapeutic effects

  • Can I reconstitute half a vial?

    While technically possible, it's not recommended. You'll need to estimate the powder amount, which introduces significant error. Peptides are manufactured and tested as complete vials.


  • What if I draw the wrong dose?

    If you realize before injecting, carefully expel the solution back into the vial and redraw the correct amount. If you've already injected, don't panic—single dose variations rarely cause issues. Simply calculate and use the correct dose going forward.

  • Do I need to recalculate if I change peptides?

    Yes, different peptides require different doses. Even if you use the same vial size and reconstitution volume, your target dose will likely differ, requiring new calculations for units to draw

Important Disclaimer:


This peptide calculator is provided for research and educational purposes only and does not constitute medical advice, diagnosis, or treatment.

Assumption of Risk: Use of this calculator and any actions you take based on its results are at your own risk. We make no warranties regarding accuracy and are not liable for any damages, injuries, or losses arising from use of this tool.

Regulatory Notice: Many peptides are for research use only and may not be FDA-approved for human use. Verify the legal status in your jurisdiction.

Individual results vary. Never disregard professional medical advice or delay seeking it because of information on this website. In case of emergency or adverse reaction, seek immediate medical attention.