Peptide Reconstitution Guide

Before you start, gather everything in a clean, organized workspace.

Tools & Supplies Needed for Peptide Reconstitution

Vials of lyophilized peptide powder

Freeze-dried peptide powder ready for reconstitution and use.

Vial of lyophilized peptide powder, a white crystalline substance inside a clear glass container with a gray cap.

Bacteriostatic water (BAC)

Preferred solvent; contains 0.9% benzyl alcohol to inhibit bacterial growth. Use BAC water for injection strictly for research purposes.

Vial of Bacteriostatic Water for Injection, USP, with pink label and cap.

Alcohol swabs

70% isopropyl swabs to maintain sterility during preparation.

Stack of individually wrapped alcohol swabs.

Insulin syringes

29–31 gauge syringes designed for precise peptide dosing.

Insulin syringe with orange cap, clear body, and measurements from 0 to 50.

Sterile mixing needles

Optional 18–21 gauge needles recommended for drawing water accurately.

Syringe, clear with a yellow needle, against a white background.

Sterile vial(s)

Used when combining or splitting peptide doses for convenience.

Box of vials, one in front. Vials are clear glass with white tops. White powder visible inside.

Peptide Safety, Hygiene & Dosage Accuracy

Calculate dosages precisely to ensure accuracy. For example, reconstituting 5 mg peptide with 2 mL BAC water means each 0.1 mL equals 0.25 mg.

Man in lab coat writing in notebook at a desk with lab equipment.
Four vials labeled

Maintain a detailed research log to track reconstitution dates, storage conditions, and observations. This helps ensure consistent and safe peptide use.

Always use sterile equipment and never reuse syringes or needles. Label all materials clearly with peptide name, reconstitution date, and concentration.

Three syringes with needles, on a light surface, ready for use.

Step-by-Step Guide to Reconstituting Peptides

1.

Prepare your workspace

Wash your hands thoroughly and wear gloves. Wipe down your surface with disinfectant.

Vial of medication, alcohol swab, and syringe on a wooden table in a room.

2.

Disinfect the vials

Use alcohol swabs to clean the rubber stoppers of both the peptide vial and BAC water vial.



3.

Draw bacteriostatic water

Use a fresh sterile needle and syringe. Slowly draw up the volume of BAC water you plan to use.

4.

Inject water into the peptide vial

Insert the needle at an angle against the glass side of the vial. Let the water gently run down the side to avoid foaming.
Hands in blue gloves hold a syringe and a vial of white powder, indoors.

5.

Dissolve the peptide

Do not shake. Gently swirl or roll the vial until the powder dissolves completely. Some peptides take longer to dissolve.


Person in gloves holding vial near laptop, possibly preparing medication at a desk.

6.

Store correctly

Most peptides should be refrigerated (2–8°C / 35–46°F).


Avoid heat, light, repeated freeze-thaw cycles, and shaking

Black case open with vials. One vial has a white label, the others are teal.

Visual Learning Resources

Female medical professional preparing medicine at a desk in a clinic, wearing scrubs and gloves.
A quick-reference spiral notebook with bullet-pointed lists lies on a table with a coffee mug, book, and lemon.

Why Proper Reconstitution Matters

Peptides are fragile chains of amino acids that degrade quickly if handled improperly. Proper reconstitution ensures maximum stability and shelf life, accurate dosing for research, and reduced contamination risk.

Reduced contamination risk

Accurate dosing for research

Maximum stability and shelf life






Legal Disclaimer:


These statements have not been evaluated by the FDA and are not intended to diagnose, treat, cure, or prevent any disease. This information is for educational and research purposes only. All products are research compounds exclusively for laboratory use under proper IRB oversight.

Peptides are not dietary supplements and are prohibited for human or animal consumption. Products must be handled only by qualified laboratory professionals trained in safe procedures and familiar with associated hazards. By accessing this site, you confirm that you are a qualified researcher using these materials solely for legitimate research purposes in compliance with applicable regulations.