Peptide Reconstitution Guide
Tools & Supplies Needed for Peptide Reconstitution
Before you start, gather everything in a clean, organized workspace.
Items for Reconstitution 1
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Vials of lyophilized peptide powderItem Link List Item 1
Freeze-dried peptide powder ready for reconstitution and use.
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Bacteriostatic water (BAC)Item Link List Item 2
Preferred solvent; contains 0.9% benzyl alcohol to inhibit bacterial growth. Use BAC water for injection strictly for research purposes.
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Alcohol swabsItem Link List Item 3
70% isopropyl swabs to maintain sterility during preparation.
Items for Reconstitution 2
Peptide Safety, Hygiene & Dosage Accuracy
Calculate dosages precisely to ensure accuracy. For example, reconstituting 5 mg peptide with 2 mL BAC water means each 0.1 mL equals 0.25 mg.
Maintain a detailed research log to track reconstitution dates, storage conditions, and observations. This helps ensure consistent and safe peptide use.
Always use sterile equipment and never reuse syringes or needles. Label all materials clearly with peptide name, reconstitution date, and concentration.
Step-by-Step Guide to Reconstituting Peptides
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1. Prepare your workspaceItem Link List Item 1
Wash your hands thoroughly and wear gloves. Wipe down your surface with disinfectant.
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2. Disinfect the vialsItem Link List Item 2
Use alcohol swabs to clean the rubber stoppers of both the peptide vial and BAC water vial.
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3. Draw bacteriostatic waterItem Link List Item 3
Use a fresh sterile needle and syringe. Slowly draw up the volume of BAC water you plan to use.
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4. Inject water into the peptide vialItem Link List Item 4
Insert the needle at an angle against the glass side of the vial. Let the water gently run down the side to avoid foaming.
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5. Dissolve the peptideItem Link
Do not shake. Gently swirl or roll the vial until the powder dissolves completely. Some peptides take longer to dissolve.
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6. Store correctlyItem Link
Most peptides should be refrigerated (2–8°C / 35–46°F).
Avoid heat, light, repeated freeze-thaw cycles, and shaking
Visual Learning Resources
After reconstituting a peptide, the most common mistake is incorrect dosing. To eliminate guesswork, use our peptide reconstitution calculator, which converts vial strength and bacteriostatic water volume into precise dosing amounts.
Why Proper Reconstitution Matters
Peptides are fragile chains of amino acids that degrade quickly if handled improperly. Proper reconstitution ensures maximum stability and shelf life, accurate dosing for research, and reduced contamination risk.
Reduced contamination risk
Accurate dosing for research
Maximum stability and shelf life
FAQ
What is the best liquid to use for peptide reconstitution?
The preferred solvent is Bacteriostatic Water (BAC). It contains 0.9% benzyl alcohol, which acts as a preservative to inhibit bacterial growth, making it safer for multi-use vials over an extended period compared to sterile water.
Why is it important not to shake the peptide vial?
Peptides are fragile chains of amino acids that can easily degrade or become "damaged" if handled roughly. Instead of shaking, you should gently swirl or roll the vial between your hands until the powder is completely dissolved to maintain the peptide's structural integrity.
How should I add the bacteriostatic water to the peptide powder?
To avoid foaming and prevent damaging the peptide, you should aim the needle toward the inside wall of the vial. Let the water run slowly down the glass side rather than injecting it directly onto the lyophilized powder.
Where should I store my peptides once they are reconstituted?
Most reconstituted peptides are highly sensitive to heat and light. They should be stored in a refrigerator at temperatures between 2–8°C (35–46°F). Keep them away from direct light and avoid repeated freeze-thaw cycles.
Can I reuse syringes or needles during the process?
No. To maintain sterility and reduce the risk of contamination, you must always use fresh, sterile equipment for every step. Never reuse syringes or needles, and ensure you disinfect the rubber stoppers of both vials with 70% isopropyl alcohol swabs before every access.
How do I determine the correct dosage after adding the water?
Dosage depends on the total milligrams (mg) of the peptide and the volume of BAC water added. For example, if you add 2 mL of water to a 5 mg vial, every 0.1 mL of liquid will contain 0.25 mg of the peptide. For more complex calculations, the guide recommends using a specialized Peptide Calculator tool.
Legal Disclaimer:
These statements have not been evaluated by the FDA and are not intended to diagnose, treat, cure, or prevent any disease. This information is for educational and research purposes only. All products are research compounds exclusively for laboratory use under proper IRB oversight.
Peptides are not dietary supplements and are prohibited for human or animal consumption. Products must be handled only by qualified laboratory professionals trained in safe procedures and familiar with associated hazards. By accessing this site, you confirm that you are a qualified researcher using these materials solely for legitimate research purposes in compliance with applicable regulations.