Step 1: Choose Your Calculation Mode

Mode 1: Find Units to Inject

Use this when: Your doctor told you to take a specific dose (e.g., "take 250 mcg")
Calculator tells you: How many units to pull on your insulin syringe

Mode 2: Find My Dose

Use this when: Your doctor told you to inject a specific number of units (e.g., "inject 20 units")
Calculator tells you: The actual dose you're getting in mcg/mg

Step 2: Enter Your Information

A. Select Syringe Size

Choose your insulin syringe size:

0.3ml = 30 units
0.5ml = 50 units
1.0ml = 100 units (most common)

B. Peptide Vial Size

Enter the amount of peptide in your vial:

Click a preset button: 5mg, 10mg, 15mg, 20mg, 50mg
OR type a custom amount in the box below

C. Bacteriostatic Water Amount

Enter how much water you're adding to reconstitute:

Click a preset: 1ml, 2ml, 3ml, 5ml
OR type a custom amount

Step 3: Enter Dose Information

If Using Mode 1 (Find Units):

Option A: Direct Dose Entry

Click a preset dose: 50mcg, 100mcg, 250mcg, 500mcg
OR type your custom dose in mcg

Option B: Body Weight-Based Dosing

Check the box: "Calculate dose based on body weight"
Enter your body weight in kg
Enter the dose per kg (your doctor should provide this)
Calculator automatically computes your total dose

If Using Mode 2 (Find Dose):

Simply enter the number of units your doctor prescribed

Step 4: Read Your Results

Mode 1 Results Show:

Your Dose: Confirms your dose in mcg
Draw Syringe To: The exact units to pull (this is what you need!)
Visual Syringe: Shows where to pull on the syringe
Concentration: How strong your mixture is (mg/ml)
Per Unit: How many mcg are in each unit

Mode 2 Results Show:

Units Prescribed: Confirms the units
Your Actual Dose: The real dose in mcg and mg
Concentration: How strong your mixture is

Per Unit: How many mcg per unit

Optional: Cycle Planning

Enter the number of days in the "Cycle Length" field to calculate:

Total peptide needed for your entire cycle
Helps you know how many vials to order

Warnings

The calculator will warn you if:

Your dose exceeds your syringe capacity
You need a larger syringe or need to adjust your water amount

Example Walkthrough

Scenario: Doctor prescribed 250 mcg of BPC-157 daily

Mode: Select "Find Units to Inject"
Syringe: Click "1.0ml" (100 units)
Vial: Click "5mg"
Water: Click "2ml"
Dose: Click "250mcg"
Result: Calculator shows "Pull to: 10 units"
Action: Draw your insulin syringe to the 10-unit mark

Quick Tips

✅ Always double-check your inputs before injecting

✅ Consult your healthcare provider with any questions

✅ Use the visual syringe as a reference guide

✅ The common peptide dosages section shows typical doses for reference

✅ If using custom amounts, make sure to enter the correct units (mg for vial, ml for water, mcg for dose)

Common Reconstitution Volumes

Recommended Reconstitution Volumes

While you can technically use any volume of bacteriostatic water, certain amounts work better for different scenarios:

2ml (Most Popular): The ideal balance for most peptides. Provides manageable concentration while keeping injection volumes reasonable. Works well for doses between 100-500mcg.
1ml (High Concentration): Best when you want minimal injection volume or are taking very small doses. Can make precise measurements more challenging for beginners.
3ml (Lower Concentration): Optimal for micro-dosing protocols or when you need very precise control over small dose adjustments. Results in larger injection volumes.

For most users working with standard 5mg vials, we recommend starting with 2ml of bacteriostatic water.

Peptide-Specific Dosing Guidelines

Use these general guidelines as starting points. Always consult with a healthcare provider before beginning any peptide protocol.

BPC-157 (Body Protection Compound)

Typical dose: 250-500mcg
Frequency: 1-2 times daily
Standard vial: 5mg

TB-500 (Thymosin Beta-4)

Typical dose: 2-2.5mg
Frequency: 2-3 times per week
Standard vial: 5mg or 10mg

Ipamorelin

Typical dose: 200-300mcg
Frequency: 2-3 times daily
Standard vial: 5mg

CJC-1295 (with or without DAC)

Typical dose: 100-200mcg
Frequency: 1-3 times per week (with DAC), daily (without DAC)
Standard vial: 2mg or 5mg

Sermorelin

Typical dose: 200-500mcg
Frequency: Once daily before bed
Standard vial: 5mg or 15mg

Note: These are general reference ranges. Individual protocols vary based on goals, body weight, and response.

Troubleshooting Common Issues

My calculator result shows more units than my syringe holds
You've likely used too much bacteriostatic water for your desired dose. Reconstitute with a smaller volume (try 1ml instead of 2ml) to create a more concentrated solution.
The numbers seem too small (2-3 units)
Very small measurements are difficult to draw accurately. Consider using less water during reconstitution to achieve a higher concentration, making your target dose correspond to a larger, more measurable number of units.
I'm getting different results from different calculators
Verify that all inputs are in the same units. Check that you're comparing calculations for the same reconstitution volume and syringe type. If discrepancies persist, work through the math manually using our formula above.
My reconstituted peptide looks cloudy
This isn't a calculation issue, but it indicates a problem. Peptide solutions should be clear. Cloudiness may indicate contamination, incorrect reconstitution, or degraded peptide. Do not use cloudy solutions.
I forgot how much water I added to my vial
Unfortunately, you cannot accurately calculate dosing without knowing the reconstitution volume. It's safer to discard the vial and start fresh with documented measurements than to risk incorrect dosing.

5. Storage and Handling Best PracticesPeptide Storage Guidelines

Proper storage is essential for maintaining peptide potency and ensuring your calculated doses remain accurate.

Before Reconstitution:
Store lyophilized peptides in the freezer (-20°C or colder)
Keep away from light and moisture
Properly stored, most peptides remain stable for 1-2 years
After Reconstitution:
Refrigerate immediately (2-8°C)
Use bacteriostatic water to extend stability
Most peptides remain potent for 28 days when properly refrigerated
Protect from light using amber vials or foil wrap
Never freeze reconstituted peptides
Label Your Vials:
Always mark your vial with:
Reconstitution date
Bacteriostatic water volume used
Concentration (mg/ml)
Units to draw per dose
Expiration date (28 days from reconstitution)

Peptide Calculator Bacteriostatic Water

What Is Bacteriostatic Water?

Bacteriostatic water is a sterile, non-pyrogenic solution that contains 0.9% benzyl alcohol as a preservative. It is commonly used for diluting or dissolving peptides and other medications intended for injection. The presence of benzyl alcohol inhibits the growth of bacteria, allowing the solution to be safely stored and reused for up to 28 days after opening.

Why It Matters in Peptide Reconstitution

When using a peptide calculator to prepare dosages, the accuracy of your results depends not only on proper measurements but also on the quality of your solvents. Bacteriostatic water is preferred over sterile water for injection when the solution will be stored and used multiple times, helping to prevent contamination and preserve peptide integrity.

Using Bacteriostatic Water with a Peptide Calculator

A peptide calculator typically requires you to input the mass of your peptide, the desired dose, and the amount of bacteriostatic water to reconstitute it with. Choosing the right amount of bacteriostatic water ensures that you achieve precise dosing and maintain peptide stability. This is especially critical when working with sensitive compounds or planning for multi-dose protocols.

Storage and Safety Tips

Storage: Always store reconstituted peptides in a refrigeration unit between 2°C and 8°C to maintain potency.
Expiration: Discard any bacteriostatic water 28 days after the vial has been opened, even if the solution appears clear and clean.
Handling: Use aseptic techniques when drawing and injecting to prevent contamination and ensure accurate dosing.

Bacteriostatic water plays an essential role in peptide preparation. With the help of a reliable peptide calculator, you can ensure accurate reconstitution, safe storage, and repeatable dosing, critical components for those working in research or clinical settings.

Project Biohacking's Peptide Calculator App

Accurate and Convenient Calculations On the Go

The Peptide Calculator app is designed to offer researchers, lab technicians, and compounders a simple yet powerful tool for calculating peptide dosages, molecular weights, dilution volumes, and reconstitution values wherever and whenever needed.

User-Friendly Interface

With an intuitive layout and streamlined navigation, the app saves time by eliminating the need for complex manual calculations. Whether you're working with milligrams, micrograms, or nanograms, the calculator adapts to your workflow efficiently.

Key Features

✔ Peptide Reconstitution: Instantly determine how much bacteriostatic water or diluent to add.

✔ Dosage Calculation: Calculate dosage per body weight, injection volume, or concentration.

✔ Unit Conversion: Seamless conversion between units such as mg/mL, IU, and more.

✔ History & Saved Sessions: View and reuse past calculations for increased productivity.

Perfect for Research and Clinical Use

Whether you’re conducting peptide-based research or preparing formulations for clinical studies, the Peptide Calculator app ensures precise results every time, reducing human error and improving consistency in your protocols.

Available for iOS and Android

Peptide Calculator Best Practices

Understanding the Importance of Accurate Calculations

When working with peptides, precision is critical. From determining molar concentrations to calculating dilution values and reconstitution volumes, a reliable peptide calculator can save time and prevent costly errors. Always begin by verifying the molecular weight and purity of your peptide, as these are essential inputs for accurate results.

Choosing the Right Peptide Calculator

Not all peptide calculators are created equal. The best tools offer flexibility to compute various parameters such as weight-to-molar conversions, reconstitution concentration, and required solvent volumes. Look for calculators that are frequently updated and backed by trusted scientific resources or manufacturers.

Input Validation Is Key

Ensure that the calculator you use allows for proper input validation. This means it should alert users to potentially incorrect or illogical entries such as incompatible units or extreme values before generating results. This feature reduces user error and enhances lab safety.

Use Verified Data

Always use verified data when inputting peptide sequences or characteristics. If available, cross-reference peptide information from your supplier’s technical datasheet.

Data integrity is vital for reproducible results, whether you're preparing stock solutions or planning in vivo studies.

Consider User Experience and Accessibility

A great peptide calculator should not only be powerful but also user-friendly. Clear interface design, mobile responsiveness, and helpful tooltips can make a big difference in day-to-day lab workflows. Accessibility helps ensure that team members at all levels of expertise can use the tool efficiently.

Conclusion.

Leveraging the best peptide calculator practices enhances the consistency and accuracy of your peptide research. By selecting reliable tools, validating inputs, and referencing accurate data, you can streamline your workflow and improve experimental outcomes.

Important Safety Information

Sterile Technique is Essential

Even with perfect calculations, improper handling can lead to contamination and serious infections. Always use:

New, sterile needles and syringes
Alcohol wipes for vial tops and injection sites
Proper hand hygiene
Clean work surfaces

When to Seek Help

Stop using peptides and contact your healthcare provider if you experience:

Severe injection site reactions (excessive swelling, redness, warmth)
Allergic reactions (rash, difficulty breathing, swelling)
Unexpected side effects
Symptoms that worsen rather than improve

Quality Matters: Source from Verified Vendors

Calculations only work when your peptides contain the labeled amount of active compound. We've vetted suppliers who provide:

Third-party testing certificates (COA)
Proper labeling and documentation
Appropriate storage during shipping
Clear expiration dates

Browse trusted peptide vendors with exclusive Project Biohacking discount codes, or compare quality standards across Limitless Biotech, Biolongevity Labs, CanLab, and Rejuvatide Peptides.

Need Help?
Have questions about using this calculator or peptide therapy in general? Contact us or consult with a qualified healthcare provider experienced in peptide therapy.

Learn More About Accurate Peptide Dosing:

Peptide Calculator: The Ultimate Guide for Accurate Dosing- Comprehensive guide covering everything from basic reconstitution to advanced protocols

Can I use regular sterile water instead of bacteriostatic water?
You can, but sterile water significantly reduces vial stability after reconstitution. With sterile water, peptides typically remain stable for only 3-5 days versus 28 days with bacteriostatic water. This means more waste and more frequent reconstitution.
What syringe size should I use?
Most peptide users rely on insulin syringes:

1ml (100 unit) syringes: Most versatile, works for most protocols

0.5ml (50 unit) syringes: Better for very small doses, offers finer gradations

0.3ml (30 unit) syringes: Best for micro-dosing protocols
How do I know if my peptides are still good?
Look for:

Clear, colorless solution (cloudiness indicates problems)

No unusual odor

Within 28 days of reconstitution

Stored properly in refrigerator

Continued therapeutic effects
Can I reconstitute half a vial?
While technically possible, it's not recommended. You'll need to estimate the powder amount, which introduces significant error. Peptides are manufactured and tested as complete vials.
What if I draw the wrong dose?
If you realize before injecting, carefully expel the solution back into the vial and redraw the correct amount. If you've already injected, don't panic—single dose variations rarely cause issues. Simply calculate and use the correct dose going forward.
Do I need to recalculate if I change peptides?
Yes, different peptides require different doses. Even if you use the same vial size and reconstitution volume, your target dose will likely differ, requiring new calculations for units to draw

Important Disclaimer:

This peptide calculator is provided for research and educational purposes only and does not constitute medical advice, diagnosis, or treatment.

Assumption of Risk: Use of this calculator and any actions you take based on its results are at your own risk. We make no warranties regarding accuracy and are not liable for any damages, injuries, or losses arising from use of this tool.

Regulatory Notice: Many peptides are for research use only and may not be FDA-approved for human use. Verify the legal status in your jurisdiction.

Individual results vary. Never disregard professional medical advice or delay seeking it because of information on this website. In case of emergency or adverse reaction, seek immediate medical attention.

Get in touch

Peptide Calculator

Peptide Reconstitution Instructions & Dosage Calculator Tool

Overview