TB -500 Dosage Calculator

How to Use the TB-500 Dosage Calculator

The calculator turns four inputs into a clean result.

Select inputs:

1. The amount of TB-500 in your vial
2. The bacteriostatic water you added to reconstitute it
3. Your target dose
4. Your syringe size

It returns the vial concentration, the exact units to draw on a U-100 insulin syringe, and how many doses the vial holds. One quirk is specific to this compound: TB-500 figures are usually written in milligrams, while the dose field here uses micrograms, so if your target is in milligrams, multiply by 1,000 first, which makes 2 mg become 2,000 mcg. Change any value and the result updates instantly, so you can compare a 5 mg and a 10 mg vial, or 1 ml against 2 ml of water, without redoing the arithmetic.

How This Calculator Works

The calculator runs three steps, and you can check each one by hand.

Concentration = vial amount ÷ water added. Draw volume = your target dose ÷ concentration. Syringe units = draw volume × 100 on a U-100 insulin syringe.

Worked in arbitrary numbers: say your target is 2,000 mcg, drawn from a 5 mg vial reconstituted with 2 ml of bacteriostatic water. Concentration is 5 mg ÷ 2 ml, which is 2.5 mg/ml, or 2,500 mcg/ml. Draw volume is 2,000 mcg ÷ 2,500 mcg/ml, which is 0.8 ml. Units are 0.8 ml × 100, which is 80 units. The 2,000 mcg here is an example input to show the arithmetic. Because TB-500 figures are usually written in milligrams, convert to micrograms first, so 2 mg becomes 2,000 mcg before entering it.



Every figure above appears in the result box, concentration, draw volume, and units side by side, so you can verify the conversion yourself rather than trusting a single number.

TB-500 is typically sold as a lyophilized powder in 2 mg, 5 mg, and 10 mg vials, and the vial size drives every downstream number. Concentration is the peptide amount divided by the bacteriostatic water you add. A 5 mg vial reconstituted with 2 ml of water gives 2.5 mg/ml, or 2,500 mcg/ml. The same 5 mg vial in 5 ml of water gives 1 mg/ml, a much thinner solution, so you would draw a larger volume for the identical dose. This is why entering your real vial size and your real water volume matters more than any preset: change either one and the units you draw change with it. For the underlying reconstitution math common to every peptide, see the main peptide calculator.

TB-500 stands apart from most research peptides in how its dose is written. Where compounds like BPC-157 are usually expressed in micrograms, TB-500 figures in research-peptide protocols are typically given in milligrams per week, often described in two stages, a higher initial period followed by a lower maintenance period. Research-peptide protocols commonly reference roughly 2 to 2.5 mg per administration, but those are community-reported figures, not established human doses, since no completed human trial of the TB-500 fragment has set a dose. This calculator does not select any figure for you. It converts a target you already have. If your source lists a weekly total, divide it into per-administration amounts, convert to micrograms, and enter that as the desired dose. For the actual research evidence behind these figures, see the TB-500 healing peptide guide.

TB-500 is a synthetic peptide based on the actin-binding fragment of thymosin beta-4, not the full thymosin beta-4 molecule. It is a short chain of amino acids studied in the context of actin regulation, tissue repair, and research on tendon, ligament, and wound healing, with an associated effect on inflammation. That mechanism is why accurate dose preparation matters to researchers working with it. This page is the calculation tool. For what TB-500 is, how it differs from full-length thymosin beta-4, and what the research actually shows, see the TB-500 healing peptide guide.

The calculator's accuracy depends entirely on the accuracy of your inputs, and two things carry the most risk. First, confirm the vial size printed on your product against what you enter, since a 2 mg vial entered as 5 mg throws off every figure. Second, watch the syringe fit: TB-500 doses run higher than many peptides, so a larger draw volume can exceed a 0.3 ml or 0.5 ml insulin syringe. When that happens, the calculator flags it, and the fix is to select a larger syringe or split the dose across two draws. The result box shows the concentration and the exact units together, so you can check the injection volume against the math rather than trusting a single number.

This calculation gives you the exact volume to draw, but accuracy depends on the right supplies and correct reconstitution. Before measuring a dose, review how to take a peptide shot for syringe setup and injection technique, and how to reconstitute peptides for the mixing steps. For the compound itself, see the TB-500 healing peptide guide.

TB-500 Dosage Calculator FAQ